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KMID : 0357419860160020151
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Korean journal of Virology
1986 Volume.16 No. 2 p.151 ~ p.158
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신영오
강춘
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Abstract
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For the antigenic analysis of rotavirus in Korea, 96 stool specimens from diarrhea patients were collected frorn 3 hospitals in two epidemic years. Rotavirus was detected from 60 specimens by ELISA (enzyme linked immunosorbent assay) using group specific monoclonal antibody. In serotyping by ELISA using subgroup specific monoclonal antibodies, Four specimens (6.7%) were subgroup I and 46 specimens (76.7%) were subgroup II. The remaining 10 specimens were belonged to intermediate. Eleven segments of RNA bands were detected from 46 specimens of 96 specimens tested (47.9%) by electrophoresis. Sixteen classes of RNA electrophoretic patterns were observed from 46 RNA positive specimens. Eighteen specimens (39.1%) were belonged to subgroup I and 27 specimens (58.7%) were subgroup II, while the remaining one (1.7%) was intermediate. Four specimens of 8 showing RNA pattern subgroup I were also belonged to ELISA subgroup I (22.2%), while 23 specimens showing RNA pattern subgroup II were belonged to ELISA subgroup II (85.2%).
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